Author(s): Yu, JH (Yu, Jiahuan); Gao, GS (Gao, Guosheng); Sun, B (Sun, Bo); Liang, LY (Liang, Lingyan); Shen, Q (Shen, Qiang); Zhang, Y (Zhang, Yang); Cao, HT (Cao, Hongtao)

Source: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS Volume: 211 Article Number: 114597 DOI: 10.1016/j.jpba.2022.114597 Published: MAR 20 2022

Abstract: In this study, separative extended-gate AlGaAs/GaAs high electron mobility transistor (HEMT) biosensor is proposed for prostate-specific antigen (PSA) detection. Zinc oxide nanotetrapods (T-ZnO) with a four-leg structure is introduced onto the sensing pad to form a three-dimensional (3D) and concave detection front. Compared with common plane front, the elaborately-designed-3D and concave detection front can offer more biological modification sites and decrease the Debye volume, resultantly improving both the detection scope and sensitivity. Anti-PSA probes can be fixed at any sites of T-ZnO via a chemical bio-functionalization method, which facilitates better detection performance by comparison with a physical modification scheme. On the other hand, the T-ZnO nanostructures with the four-leg configuration are capable of re-leasing the stress and erosion effect of the solution on the plane Au film, contributing to a great improvement in the reliability of the biosensors. The optimized biosensors with chemical bio-functionalized T-ZnO detection front demonstrate good linear current/voltage response to label-free PSA target in the concentration range from 5 fg/ml similar to 5 ng/ml and a sensitivity variation similar to 1.3% dec(-1). (C) 2022 Elsevier B.V. All rights reserved.

Accession Number: WOS:000762677500003

PubMed ID: 35074564

ISSN: 0731-7085

eISSN: 1873-264X

Full Text: https://www.sciencedirect.com/science/article/pii/S0731708522000188?via%3Dihub