Author(s): Li, YM (Li, Yamin); Zhan, LJ (Zhan, Lijie); Wang, Y (Wang, Yang); Chen, RR (Chen, Ruru); Yang, XW (Yang, Xiaowei); Wu, XT (Wu, Xiaoting); Wang, YJ (Wang, Yijun); Chen, HD (Chen, Hongda); Xu, C (Xu, Chun); Pei, WH (Pei, Weihua)

Source: SENSORS AND ACTUATORS A-PHYSICAL Volume: 331 Article Number: 112948 DOI: 10.1016/j.sna.2021.112948 Published: NOV 1 2021

Abstract: Calcium (Ca2+) fluorescence is widely used to monitor the activity of neurons in vivo. We propose a method of detection the Ca2+ fluorescence intensity by integrating a mu LED linear array and an optical fiber. Due to the different positions of each mu LED, the range of excitation of each mu LED cannot overlap that of another by controlling the luminous intensity. When each mu LED in a linear array is switched on in turn, only one mu LED is emitting at any given time. In this way, the fluorescence from the active neurons collected by a single fiber can correspond to the position of the mu LED according to the time sequence of each mu LED, thus resulting in the relative position of the recorded fluorescence being distinguished. When the scanning speed of a mu LED is much higher than the fluorescence process associated with Ca2+ activity, the device can simultaneously obtain the Ca2+ fluorescence activity signals from multiple sites, thus inferring the activity of neurons through the fluorescence intensity, so that the neuron activity with depth resolution can be obtained. In the present work, the prototype device and system are constructed, and the method has been verified by simulation in vitro and green fluorescent protein (GFP) in vivo. (C) 2021 Elsevier B.V. All rights reserved.

Accession Number: WOS:000702537600002

ISSN: 0924-4247

eISSN: 1873-3069

Full Text: https://www.sciencedirect.com/science/article/pii/S0924424721004131?via%3Dihub