Author(s): Luo, JM (Luo, Jiaming); Li, SF (Li, Sufang); Xu, MK (Xu, Mengke); Guan, M (Guan, Min); Yang, MX (Yang, Mengxi); Ren, JY (Ren, Jingyi); Zhang, Y (Zhang, Yang); Zeng, YP (Zeng, Yiping)

Source: AIP ADVANCES Volume: 10 Issue: 11 Article Number: 115205 DOI: 10.1063/5.0027553 Published: NOV 1 2020

Abstract: The high electron mobility transistor (HEMT)-based biosensors are highly competitive in the ultimate application of portable and point-of-care testing. Herein, we have demonstrated highly sensitive and real-time detection of cardiac troponin I (cTnI), a biomarker for the diagnosis of acute myocardial infarction (AMI) using AlGaAs/GaAs HEMT-based biosensors. The device has achieved a lower detection limit of 1 pg/ml in the buffer solution and less than 30 s response time, which demonstrated significant promise in the early diagnosis and screening of AMI. In addition, our results are consistent with the enzyme-linked immunosorbent assay according to the AMI patient's blood test results. Furthermore, by comparing the two HEMT structures, we also calculated the equilibrium dissociation constant (K-D) of the cTnI and cTnI antibody and analyzed the sensing mechanism. The results show that this method is very promising for early diagnosis of AMI.

Accession Number: WOS:000591287400001

eISSN: 2158-3226

Full Text: https://aip.scitation.org/doi/10.1063/5.0027553