Authors: Wang, SC; Lv, XQ; Su, Y; Fan, ZY; Fang, WH; Duan, JZ; Zhang, S; Ma, BJ; Liu, F; Chen, HD; Geng, ZX; Liu, H
SMALL
Volume: 15 Issue: 9 Published: MAR 1 2019 Language: English Document type: Article
DOI: 10.1002/smll.201804593
Abstract:
Cell lysis is an important and crucial step for the detection of intracellular secrets. Usually, cell lysis is based on strong ultrasonic waves or toxic chemical regents, which require a large amount of cell suspension. To obtain high efficiency cell lysis for a small amount of sample, a mechanical cell lysis method based on a surface acoustic wave (SAW) microchip is proposed. The microchip simply consists of a piece of LiNbO3 crystal substrate, interdigitated transducers (IDTs) with 80 pairs of parallel electrodes and 3M Magic Tapes. The modulated input electrical signal is coupled into the substrate through IDTs, which produces an acoustic stream in the droplet on the surface of a substrate. When a biofluid droplet containing cells and microparticles is dropped on the surface of the microchip, the cells and microparticles are accelerated and collide with each other. The fluorescence staining results illustrate that the cell membrane is efficiently destroyed and that proteins as well as nucleic acids inside the cell are released. The experimental results show that this method has a high efficiency and low sample consumption. The potential application is the pretreatment of a small amount of tested sample in a hospital or biolab.
全文链接：https://onlinelibrary.wiley.com/doi/10.1002/smll.201804593