Author(s): Lv, XQ (Lv, Xiaoqing); Ma, ZT (Ma, Zhengtai); Guo, L (Guo, Lin)

Source: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY Volume: 87  Issue: 12 Pages: 1478-1484  DOI: 10.1093/bbb/zbad123  Early Access Date: SEP 2023  Published Date: 2023 NOV 21 

Abstract: Developing cell cryopreservation methods on chips is not only crucial for biomedical science but also represents an innovative approach for preserving traditional cell samples. This study presents a simple method for direct cell freezing and thawing on chip, allowing for long-term storage of cells. During the freezing process, cells were injected into the microchannel along with a conventional cell cryopreservation solution, and the chip was packed using a self-sealing bag containing isopropyl alcohol and then stored in a -80 degrees C refrigerator until needed. During the thawing process, microcolumn arrays with a spacing of 8 mu m were strategically incorporated into the microfluidic chip design to effectively inhibit cells from the channel. The breast cancer cell lines MDA-MB-231 and B47D demonstrated successful thawing and growth after cryopreservation for 1 month to 1 year. These findings offer a direct cell freezing and thawing method on a microfluidic chip for subsequent experiments.

Graphical Abstract The simple and time-saving cell freezing and thawing method on a microfluidic device.

Accession Number: WOS:001184160300001

PubMed ID: 37660248

ISSN: 0916-8451

eISSN: 1347-6947